Advanced Research Internship Program 2020

Dates

Time (Pacific)

Time (Eastern)

Activities

Sa 20 Jun

2p – 3p

5p – 6p

Family welcome and introduction

Su 28 Jun

(Sign up for individual times)

Pre-internship computer/internet check and field sampling design
(individual conferences with each intern and family)

Week 1: Begin individual work and perform field sampling

Mo 6 July

8a – 2p

11a–5p

Introductions and unboxing of ARIP kit

Communications: Slack, Google Drive, collecting protocol intro

iNaturalist introduction, collecting locations [RW]

Introduction to drafting a CV (resumé) & personal statement [RW]

Collecting protocol details: field notes, photos, and tissue sampling

Tu 7 July

Specimen collection and photography in local ecosystems

Specimen acquisition at local fish market (crab & fish & strawberries)

Curriculum vitae (CV) preparation & upload personal statement

We 8 July

9a

12p

Check-in/progress reports

Discuss eDNA collecting protocol intro [RW]

Read Taberlet Chapter 1 — Introduction to environmental DNA

Th 9 July

9a

12p

Check-in/progress reports

Test Geneious sequence editing software (after 8 July: begin 14-day “trial license”)

Introduction to Geneious DNA sequence editing software [AW]

Begin editing L.A. Urban Ocean Expedition (LAUOE) sequences

Ship tissue samples to Los Angeles

Fr 10 July

8a

11a

First round of CV content review

Taberlet chapter discussion and questions

LAUOE sequence editing continues

Sa 11 July

Day off!

Week 2: Biodiversity, environmental DNA, and working in science

Su 12 July

Day off!

Mo 13 July

8a – 5p

11a – 8p

NHMLA’s DISCO project overview [NDP]

Set up fairy shrimp hatching [AW]

Sequence editing of LAUOE sequences (Check in on sample data 3) [AW]

DNA, molecular biology, and barcode genes [JW]

Evans & Paulay paper discussion

Single species detection (qPCR, etc.) — Taberlet “Single species detection” paper

[homework: read Wall et al. qPCR manuscript, Olson, 2020, and view AAAS video]

Tu 14 July

8a – 5p

11a – 8p

Introduction to scientific communication (ABT) [NDP]

Finding peer-reviewed literature [NDP]

Management of a literature database using Zotero [NDP]

[11a/2p] Behind the scenes tours of the NHMLA — Crustacea [AW, JW]

Selecting research/presentation topics

Introduction to fairy shrimp [AW]

[homework: begin work on research presentation topic, view ABT builds video]

[homework: Qiagen extraction kit - read overview and pgs. 32-35]

[defrost frozen crab]

We 15 July

8a – 5p

11a – 8p

[8a/11a] Evolutionary trees [RW]

Qiagen extraction

Biodiversity

Evolutionary trees (Palumbi whale forensics in Japan paper) [RW]

Curriculum vitae (CV) refinements

DNA extraction (cheek swab or strawberry) [AW, JW]

Continuing research work for research presentation

Live streamed steamed Crustacea dissection [AW]

Th 16 July

8a – 5p

11a – 8p

Check-in on research progress

Introduction to international sequence repositories (BOLD and GenBank) [AW]

Continuing research work for research presentation

Check-in on research progress

[homework: refining CV for college application]

[homework: read qPCR peer review comments]

Fr 17 July

8a – 5p

11a – 8p

[10a/1p] Behind the scenes tours of the NHMLA — Ichthyology [Todd Clardy]

Refining CVs for college application

Examine qPCR manuscript peer review comments

Continuing research work for research presentation: Dobzhansky sentence

[homework: develop ABT]

Sa 18 July

8a – 5p

11a – 8p

Refining CVs for college application

Paddlefish & Sturgeon hybrids (What is a species?)

[homework during the day: ABT work]

ABT refinement as a group

[homework: presentation outline]

Week 3: Data posting to BOLD, research work completion, and presentations

Su 19 July

12:30p – 5p

3:30p – 8p

Working on research presentation as a group

Specimen information and photo posting to Barcode of Life Database [NDP]

[homework: continuing work on research presentation]

Mo 20 July

8a – 5p

11a – 8p

Working on research presentations as a group

[11a/2p] Fish printing

Working on research presentations as a group

Tu 21 July

8a – 5p

11a – 8p

Complete research presentation

Complete refining CVs for college application

DNA sequence and trace file posting to Barcode of Life Database [NDP, AW]

We 22 July

9a – 10:30a

11a – 11:30a

11:30p

12p – 1:30p

2p – 2:30p

2:30p

Final research presentations

Guidance on application letters and CV text describing ARIP [RW]

[11:30a/2:30p] Closing ceremony